Analytical expertise in (Epi)Lipidomics

Lipid fractionation / enrichment - 7 members

Description	Scientist	Laboratory	City
Yes	Andrew Pitt	The University of Manchester, UK
solid-phase extraction protocols	Ana Reis	FoodPhenolab@REQUIMTE	Porto
YES	Julia Kuligowski	Health Research Institute La Fe, Valencia, Spain	València
not really	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
isolation of cellular compartments	Maria Balazova	Centre of Biosciences, Institute of Animal Biochemistry and Genetics
SPE	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences
SPE, liquid-liquid extraction	Valery Bochkov	Valery Bochkov

Lipid extraction - 11 members

Description	Scientist	Laboratory	City
liquid-liquid extraction procedures	Ana Reis	FoodPhenolab@REQUIMTE	Porto
From plants and fungi	Carmit Ziv	Postharvest Pathology, ARO	Rishon LeTsiyon
various methods with different optimised solvents MTBE, CH2Cl2 ...	Georgios Theodoridis	Biomic AUTh, CIRI and Laboratory of Analytical Chemistry, Aristotle Universit, Thessaloniki GREECE
PUFAs	Helike Lõhelaid	HiLIFE/Neuroscience Center, University of Helsinki, Finland	Helsinki
YES	Julia Kuligowski	Health Research Institute La Fe, Valencia, Spain	València
yes LLE and SPE	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
chloroform:methanol	Maria Balazova	Centre of Biosciences, Institute of Animal Biochemistry and Genetics
LLE (MTBE mainly)	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences
Folch	Valery Bochkov	Valery Bochkov
Folch, MTBE	Yesim Er Oztas	Yesim Er Oztas
MTBE, Folch, Folch, Bligh-Dyer	Zhixu Ni	Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of the Technical University Dresden	Dresden

Gas chromatography (GC) - 9 members

Description	Scientist	Laboratory	City
Yes	Andrew Pitt	The University of Manchester, UK
For sterols	Carmit Ziv	Postharvest Pathology, ARO	Rishon LeTsiyon
GC	Georgios Theodoridis	Biomic AUTh, CIRI and Laboratory of Analytical Chemistry, Aristotle Universit, Thessaloniki GREECE
YES	Julia Kuligowski	Health Research Institute La Fe, Valencia, Spain	València
yes	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Inna Khozin-Goldberg	The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev	Midreshet Ben-Gurion
yes	Maria Balazova	Centre of Biosciences, Institute of Animal Biochemistry and Genetics
GC-MS Fatty acid analysis	Rosário Domingues	Lipidomic Lab, Mass Spectrometry Center, Department of Chemsitry, University of Aveiro, 3810-193 Aveiro, Portugal
only for FAMEs	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences

Hydrophilic interaction liquid chromatography (HILIC) - 6 members

Description	Scientist	Laboratory	City
Yes	Andrew Pitt	The University of Manchester, UK
HILIC	Georgios Theodoridis	Biomic AUTh, CIRI and Laboratory of Analytical Chemistry, Aristotle Universit, Thessaloniki GREECE
YES	Julia Kuligowski	Health Research Institute La Fe, Valencia, Spain	València
yes	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
HILIC-LC-MS	Rosário Domingues	Lipidomic Lab, Mass Spectrometry Center, Department of Chemsitry, University of Aveiro, 3810-193 Aveiro, Portugal
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences

Reverse phase liquid chromatography (RPLC) - 10 members

Description	Scientist	Laboratory	City
Yes	Andrew Pitt	The University of Manchester, UK
RPLC	Georgios Theodoridis	Biomic AUTh, CIRI and Laboratory of Analytical Chemistry, Aristotle Universit, Thessaloniki GREECE
Oxylipins	Helike Lõhelaid	HiLIFE/Neuroscience Center, University of Helsinki, Finland	Helsinki
YES	Julia Kuligowski	Health Research Institute La Fe, Valencia, Spain	València
yes	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Inna Khozin-Goldberg	The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev	Midreshet Ben-Gurion
C18 RP-LC-MS	Rosário Domingues	Lipidomic Lab, Mass Spectrometry Center, Department of Chemsitry, University of Aveiro, 3810-193 Aveiro, Portugal
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences
C18	Valery Bochkov	Valery Bochkov
C18, C30	Zhixu Ni	Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of the Technical University Dresden	Dresden

Thin layer chromatography (TLC) - 11 members

Description	Scientist	Laboratory	City
2D TLC	Ana Reis	FoodPhenolab@REQUIMTE	Porto
For CerS activity assay	Carmit Ziv	Postharvest Pathology, ARO	Rishon LeTsiyon
Oxylipins, PUFAs	Helike Lõhelaid	HiLIFE/Neuroscience Center, University of Helsinki, Finland	Helsinki
yes a bit stil usefull	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Inna Khozin-Goldberg	The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev	Midreshet Ben-Gurion
yes	Maria Balazova	Centre of Biosciences, Institute of Animal Biochemistry and Genetics
TLC	Rosário Domingues	Lipidomic Lab, Mass Spectrometry Center, Department of Chemsitry, University of Aveiro, 3810-193 Aveiro, Portugal
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences
Silicagel	Valery Bochkov	Valery Bochkov
TLC od polar and nonpolar lipids of the erythrocyte	Yesim Er Oztas	Yesim Er Oztas
NP-TLC, RP-TLC	Zhixu Ni	Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of the Technical University Dresden	Dresden

Capillary electrophoresis - 1 member

Description	Scientist	Laboratory	City
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse

Normal phase liquid chromatography (NPLC) - 3 members

Description	Scientist	Laboratory	City
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Inna Khozin-Goldberg	The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev	Midreshet Ben-Gurion
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences

Other - 1 member

Description	Scientist	Laboratory	City
SFC	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse

Imaging MS - 3 members

Description	Scientist	Laboratory	City
Yes	Andrew Pitt	The University of Manchester, UK
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
no	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences

Ion mobility-MS - 5 members

Description	Scientist	Laboratory	City
Yes	Andrew Pitt	The University of Manchester, UK
IMS	Georgios Theodoridis	Biomic AUTh, CIRI and Laboratory of Analytical Chemistry, Aristotle Universit, Thessaloniki GREECE
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences
Travelling Wave Ion Mobility	Zhixu Ni	Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of the Technical University Dresden	Dresden

LC-MS - 7 members

Description	Scientist	Laboratory	City
Yes	Andrew Pitt	The University of Manchester, UK
LCMS	Georgios Theodoridis	Biomic AUTh, CIRI and Laboratory of Analytical Chemistry, Aristotle Universit, Thessaloniki GREECE
Oxylipins	Helike Lõhelaid	HiLIFE/Neuroscience Center, University of Helsinki, Finland	Helsinki
YES	Julia Kuligowski	Health Research Institute La Fe, Valencia, Spain	València
yes	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences
Orbitrap, QToF	Zhixu Ni	Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of the Technical University Dresden	Dresden

Lipid identification - 5 members

Description	Scientist	Laboratory	City
Yes	Andrew Pitt	The University of Manchester, UK
YES	Julia Kuligowski	Health Research Institute La Fe, Valencia, Spain	València
yes a bit	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences
LipidHunter, LPPtiger, Lipostar, MS-DIAL, LipidXplorer	Zhixu Ni	Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of the Technical University Dresden	Dresden

Lipid quantification - 5 members

Description	Scientist	Laboratory	City
Yes	Andrew Pitt	The University of Manchester, UK
YES	Julia Kuligowski	Health Research Institute La Fe, Valencia, Spain	València
yes	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences
Lipostar, MS-DIAL	Zhixu Ni	Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of the Technical University Dresden	Dresden

Lipid standards - 3 members

Description	Scientist	Laboratory	City
Yes	Andrew Pitt	The University of Manchester, UK
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences

Shotgun MS - 3 members

Description	Scientist	Laboratory	City
Yes	Andrew Pitt	The University of Manchester, UK
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
Nanomate + LTQ-Orbitrap	Zhixu Ni	Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of the Technical University Dresden	Dresden

Targeted lipidomics (SRM, MRM, PRM) - 5 members

Description	Scientist	Laboratory	City
Yes	Andrew Pitt	The University of Manchester, UK
SRM	Georgios Theodoridis	Biomic AUTh, CIRI and Laboratory of Analytical Chemistry, Aristotle Universit, Thessaloniki GREECE
YES	Julia Kuligowski	Health Research Institute La Fe, Valencia, Spain	València
yes (MRM) and semi targeted	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
MRM, PRM	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences

Untargeted data dependent acquisition (DDA) - 7 members

Description	Scientist	Laboratory	City
Yes	Andrew Pitt	The University of Manchester, UK
DDA	Georgios Theodoridis	Biomic AUTh, CIRI and Laboratory of Analytical Chemistry, Aristotle Universit, Thessaloniki GREECE
YES	Julia Kuligowski	Health Research Institute La Fe, Valencia, Spain	València
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
DDA	Rosário Domingues	Lipidomic Lab, Mass Spectrometry Center, Department of Chemsitry, University of Aveiro, 3810-193 Aveiro, Portugal
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences
generic DDA	Zhixu Ni	Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of the Technical University Dresden	Dresden

Untargeted data independent acquisition (DIA) - 5 members

Description	Scientist	Laboratory	City
Using Synapt	Carmit Ziv	Postharvest Pathology, ARO	Rishon LeTsiyon
DIA	Georgios Theodoridis	Biomic AUTh, CIRI and Laboratory of Analytical Chemistry, Aristotle Universit, Thessaloniki GREECE
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences
MSe	Zhixu Ni	Center of Membrane Biochemistry and Lipid Research, University Hospital and Faculty of Medicine Carl Gustav Carus of the Technical University Dresden	Dresden

Proteomics - 5 members

Description	Scientist	Laboratory	City
Yes	Andrew Pitt	The University of Manchester, UK
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Inna Khozin-Goldberg	The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev	Midreshet Ben-Gurion
yes, core facility	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences
MS	Valery Bochkov	Valery Bochkov

Genomics - 4 members

Description	Scientist	Laboratory	City
For fungi	Carmit Ziv	Postharvest Pathology, ARO	Rishon LeTsiyon
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Inna Khozin-Goldberg	The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev	Midreshet Ben-Gurion
Microarrays	Valery Bochkov	Valery Bochkov

Transcriptomics - 5 members

Description	Scientist	Laboratory	City
For plants and fungi	Carmit Ziv	Postharvest Pathology, ARO	Rishon LeTsiyon
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Inna Khozin-Goldberg	The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev	Midreshet Ben-Gurion
yes, external sources	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences
genes related to lipids function/metabolism	Sophia Letsiou	University of West Attica	Athina

1 member

Description	Scientist	Laboratory	City
Protein pull-down assays using liposomes	Valery Bochkov	Valery Bochkov

Fluorescence spectroscopy - 4 members

Description	Scientist	Laboratory	City
steady-state fluorescence	Ana Reis	FoodPhenolab@REQUIMTE	Porto
Fluorescence quenching	Catarina Pereira-Leite	CBIOS, Universidade Lusófona, Lisbon, Portugal	Lisboa
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
steady-state & time-resolved	Maria Sarmento	Instituto de Medicina Molecular	Lisboa

Fluorescence microscopy - 6 members

Description	Scientist	Laboratory	City
For fungal samples	Carmit Ziv	Postharvest Pathology, ARO	Rishon LeTsiyon
yes	Ines Drenjancevic	Faculty of Medicine Osijek, University of Osijek	Osijek
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Inna Khozin-Goldberg	The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev	Midreshet Ben-Gurion
Confocal, FRET, FLIM, FCS, STED	Maria Sarmento	Instituto de Medicina Molecular	Lisboa
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences

UV/Vis spectroscopy - 4 members

Description	Scientist	Laboratory	City
Derivative spectrophotometry	Catarina Pereira-Leite	CBIOS, Universidade Lusófona, Lisbon, Portugal	Lisboa
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Inna Khozin-Goldberg	The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev	Midreshet Ben-Gurion
Various colorimetric assays	Yesim Er Oztas	Yesim Er Oztas

Others - 1 member

Description	Scientist	Laboratory	City
X-ray diffraction, calorimetry	Catarina Pereira-Leite	CBIOS, Universidade Lusófona, Lisbon, Portugal	Lisboa

Raman spectroscopy - 2 members

Description	Scientist	Laboratory	City
YES	Julia Kuligowski	Health Research Institute La Fe, Valencia, Spain	València
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse

Western blot - 8 members

Description	Scientist	Laboratory	City
Yes	Carmit Ziv	Postharvest Pathology, ARO	Rishon LeTsiyon
Lipoxygenases, cyclooxygenases	Helike Lõhelaid	HiLIFE/Neuroscience Center, University of Helsinki, Finland	Helsinki
yes	Ines Drenjancevic	Faculty of Medicine Osijek, University of Osijek	Osijek
YES	Julia Kuligowski	Health Research Institute La Fe, Valencia, Spain	València
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Inna Khozin-Goldberg	The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev	Midreshet Ben-Gurion
yes	Maria Balazova	Centre of Biosciences, Institute of Animal Biochemistry and Genetics
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences

ELISA - 6 members

Description	Scientist	Laboratory	City
AA derived oxylipins (HETEs, prostaglandins)	Helike Lõhelaid	HiLIFE/Neuroscience Center, University of Helsinki, Finland	Helsinki
yes	Ines Drenjancevic	Faculty of Medicine Osijek, University of Osijek	Osijek
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Maria Balazova	Centre of Biosciences, Institute of Animal Biochemistry and Genetics
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences
Antibodies to oxidized PLs	Valery Bochkov	Valery Bochkov

Microarrays - 2 members

Description	Scientist	Laboratory	City
no	Justine BERTRAND-MICHEL	MetaToul-Lipidomic	Toulouse
yes	Ondrej Kuda	Institute of Physiology, Czech Academy of Sciences

1 member

Description	Scientist	Laboratory	City
Fluorescence and AFM-based approaches	Maria Sarmento	Instituto de Medicina Molecular	Lisboa

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Sample preparation

Separation techniques

Mass Spectrometry

Lipidomics workflows

Other omics technologies

Lipid-protein interactions

Spectroscopy

Immune assay for lipid analysis

Biophysical methods

CA19105 – Pan-European Network in Lipidomics and EpiLipidomics